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Goat Anti-VPS18 Antibody (EB06829)

Related Products: EBP06829 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06829 Goat Anti-VPS18 Antibody Pep-ELISA Allow up to 10 days for delivery 100µg specific antibody in 200µl $255.00 Aspiring

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: VPS18, vacuolar protein sorting protein 18, HGNC:15972, KIAA1475, vacuolar protein sorting 18, vacuolar protein sorting 18 homolog (S. cerevisiae), PEP3
Official Symbol: VPS18
Accession Number(s): NP_065908.1
Human GeneID(s): 57617

Immunogen

Peptide with sequence CADLPEEDEELRKK, from the internal region of the protein sequence according to NP_065908.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 25-26kDa band in lysates of Human Duodenum, Colon and Placenta after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 110kDa according to NP_065908.1. The 25-26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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