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Goat Anti-VPS29 (C Terminus) Antibody (EB06257)

Related Products: EBP06257 - Immunizing Peptide, EBP07369 - Immunizing Peptide, Goat Anti-VPS29 (Internal) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06257 Goat Anti-VPS29 (C Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
www.arp1.com
EB06257 1
EB06257 staining (0.1µg/ml) of Human Spleen lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: VPS29, DC7, DC15, PEP11, FLJ20492, DKFZp564F0223, vacuolar protein sorting 29 (yeast), x 007 protein, retromer protein, vacuolar sorting protein VPS29/PEP11, vacuolar protein sorting 29 (yeast homolog), vacuolar protein sorting 29 homolog (S. cerevisiae), vacuolar protein sorting 29
Official Symbol: VPS29
Accession Number(s): NP_057310.1; NP_476528.1
Human GeneID(s): 51699
Non-Human GeneID(s): 56433 (mouse)
Important Comments: This antibody is expected to recognise both human isoforms (represented by NP_057310.1; NP_476528.1).

Immunogen

Peptide with sequence C-DDVKVERIEYKKP, from the C Terminus of the protein sequence according to NP_057310.1; NP_476528.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 20+21kDa bands observed in Human Spleen lysates (calculated MW of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubated for 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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