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Goat Anti-VPS33B (internal) Antibody (EB06827)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06827 | Goat Anti-VPS33B (internal) Antibody | Pep-ELISA | Allow up to 10 days for delivery | 100µg specific antibody in 200µl | $255.00 |  |
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Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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Target Protein
Principal Names: VPS33B, vacuolar protein sorting 33B (yeast), HGNC:12712, FLJ14848, vacuolar protein sorting 33B (yeast homolog), vacuolar protein sorting 33B (yeast homolog)Official Symbol: VPS33B
Accession Number(s): NP_061138.2
Human GeneID(s): 26276
Immunogen
Peptide with sequence CESIMKKKTKQDFQ, from the internal region of the protein sequence according to NP_061138.2.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Western Blot: Preliminary experiments gave an approx 48kDa band in Human lysates of kidney fibroblast cell line HEK293 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.6kDa according to NP_061138.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Immunohistochemistry: Preliminary experiments in Human Kidney showed staining of epithelial cells of renal glomeruli and some renal tubules. Recommended concentration 5-10µg.
Species Reactivity
Tested:Expected from sequence similarity: Human, Mouse, Rat, Yeast