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|EB06268-T||Goat Anti-VPS35 / MEM3 Antibody - Trial||Pep-ELISA, WB, IF||20µg specific antibody in 40µl||Human, Mouse||$105.00|
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Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.
This antibody (previous batch) has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
This antibody (previous batch) has been successfully used in ICC on Human:
Kanatsu K, Hori Y, Ebinuma I, Chiu YW, Tomita T.
Retrograde transport of γ-secretase from endosomes to the trans-Golgi network regulates Aβ42 production.
J Neurochem. 2018 May 31.
This antibody (previous batch) has been successfully used in IF on Human:
Takatori S, Tatematsu T, Cheng J, Matsumoto J, Akano T, Fujimoto T.
Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes
Traffic. 2016 Feb;17(2):154-67
This antibody (previous batch) has been successfully used in IF on CHO cells:
Lee S, Uchida Y, Emoto K, Umeda M, Kuge O, Taguchi T, Arai H.
Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis.
Genes Cells. 2012 Aug;17(8):728-36. doi: 10.1111/j.1365-2443.2012.01622.x.