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Goat Anti-VRK2 Antibody (EB09545)

Related Products: EBP09545 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09545 Goat Anti-VRK2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09545 1
EB09545 (2µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: VRK2, vaccinia related kinase 2, vaccinia virus B1R-related kinase 2, vaccinia-related kinase-2
Official Symbol: VRK2
Accession Number(s): NP_001129499.1; NP_006287.2; NP_001123954.1; NP_001123955.1
Human GeneID(s): 7444
Important Comments: This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2.

Immunogen

Peptide with sequence C-PNSQKVDSQKAATK, from the internal region of the protein sequence according to NP_001129499.1; NP_006287.2; NP_001123954.1; NP_001123955.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 60kDa band observed in nuclear lysates of cell line K562 (calculated MW of 58.1kDa according to NP_001123954.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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