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Goat Anti-WNT15 / WNT9B Antibody (EB09452)

Code Name Applications Availability Size Tested species Price Grade  
EB09452 Goat Anti-WNT15 / WNT9B Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB09452 1
EB09452 (0.3µg/ml) staining of Human Brain Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09452 2
EB09452 (1µg/ml) staining of Mouse Kidney (A) and Brain (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: WNT9B, wingless-type MMTV integration site family, member 9B, WNT14B, WNT15, wingless-type MMTV integration site family, member 15
Official Symbol: WNT9B
Accession Number(s): NP_003387.1
Human GeneID(s): 7484
Non-Human GeneID(s): 22412 (mouse), 303586 (rat)

Immunogen

Peptide with sequence C-KRGNKDLRARADA, from the internal region of the protein sequence according to NP_003387.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 38kDa band observed in Human Brain Cerebellum lysates and in Mouse Kidney and Brain lysates (calculated MW of 39kDa according to NP_003387.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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