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Goat Anti-YAP1 Antibody (EB07919)

Related Products: EBP07919 - Immunizing Peptide, EBP09727 - Immunizing Peptide, Goat Anti-YAP / YAP1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07919 Goat Anti-YAP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Dog $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07919 1
EB07919 (0.5µg/ml) staining of MDCK lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Yes-associated protein 1, 65kDa, YAP, YAP2, YAP65, Yes-associated protein 1, 65 kD, Yes-associated protein 1, 65 kDa, yes-associated protein 2
Official Symbol: YAP1
Accession Number(s): NP_006097.1
Human GeneID(s): 10413
Non-Human GeneID(s): 22601 (mouse), 363014 (rat)

Immunogen

Peptide with sequence C-TYHSRDESTDS, from the internal region of the protein sequence according to NP_006097.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 70kDa band observed in lysates of cell line MDCK (calculated MW of 50.3kDa according to Dog XP_536601.2). Data obtained from an anonymous customer. Recommended concentration: 0.5-2µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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