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Goat Anti-ARPC2 Antibody (EB05433)

Related Products: EBP05433 - Immunizing Peptide, Goat Anti-ARPC2 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05433 Goat Anti-ARPC2 Antibody Pep-ELISA, IF, IHC, WB Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB05433 1
EB05433 (0.5µg/ml) staining of CACO-2 (A), (0.1ug/ml) HeLa (B) and Jurkat (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05433 2
EB05433 (3.8µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05433 3
EB05433 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05433 4
EB05433 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
Official Symbol: ARPC2
Accession Number(s): NP_005722.1; NP_690601.1
Human GeneID(s): 10109
Non-Human GeneID(s): 76709 (mouse)
Important Comments: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Immunogen

Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1; NP_690601.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 34-35kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3kDa according to NP_005722.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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