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Code Name Applications Availability Size Tested species Price Grade  
EB07174 Goat Anti-AIF1 / IBA1 (isoform 3) Antibody Pep-ELISA, FC, IF, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07174 1
EB07174 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07174 2
EB07174 Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07174 3
EB07174 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07174 4
EB07174 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml) .IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07174 5
EB07174 (6µg/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB07174 6
EB07174 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.
EB07174 7
EB07174 (5µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07174 8
EB07174 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
Official Symbol: AIF1
Accession Number(s): NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse)
Important Comments: This antibody is expected to recognise isoform 3 (NP_001614.3) only.

Immunogen

Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

Specific Reference

This antibody (previous batch) has been successfully used in IHC on Rat:
Joshua P. Thaler, Chun-Xia Yi, Ellen A. Schur, Stephan J. Guyenet, Bang H. Hwang, Marcelo O. Dietrich, Xiaolin Zhao, David A. Sarruf, Vitaly Izgur, Kenneth R. Maravilla, Hong T. Nguyen, Jonathan D. Fischer, Miles E. Matsen, Brent E. Wisse, Gregory J. Morton, Tamas L. Horvath, Denis G. Baskin, Matthias H. Tschöp, and Michael W. Schwartz
Obesity is associated with hypothalamic injury in rodents and humans.
J Clin Invest. 2012 Jan 3; 122(1): 153-162.
PMID: 22201683

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