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Code Name Applications Availability Size Tested species Price Grade  
EB07258 Goat Anti-Doublecortex / DCX Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07258 1
EB07258 (0.003µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07258 2
EB07258 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07258 3
EB07258 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
EB07258 4
EB07258 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: DCX, doublecortex, lissencephaly, X-linked (doublecortin), RP5-914P14.1, DBCN, DC, LISX, SCLH, XLIS, OTTHUMP00000062892, doublecortin, doublin, lissencephalin-X
Official Symbol: DCX
Accession Number(s): NP_000546.2; NP_835365.1; NP_835364.1; NP_835366.1
Human GeneID(s): 1641
Important Comments: This antibody is expected to recognise isoform a (NP_000546.2), isoform b (NP_835365.1), and isoform c (NP_835364.1 and NP_835366.1).

Immunogen

Peptide with sequence C-KASPTPQKTSAKSPG, from the internal region of the protein sequence according to NP_000546.2; NP_835365.1; NP_835364.1; NP_835366.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to Mouse NP_001103692.1). Recommended concentration: 0.003-0.01µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 7µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human; Mouse, Rat, Dog, Cat

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