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Goat Anti-Alpha-synuclein Antibody (EB11713)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB11713 | Goat Anti-Alpha-synuclein Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human, Mouse | $411.00 |  |
|
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OR
Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: alpha-synuclein, NACP, non A-beta component of AD amyloid, PARK1, PARK4, PD1, SNCA, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor)Official Symbol: SNCA
Accession Number(s): NP_009292.1; NP_000336.1
Human GeneID(s): 6622
Non-Human GeneID(s): 20617 (mouse), 29219 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_009292.1; NP_000336.1). Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1
Immunogen
Peptide with sequence C-ATGFVKKDQLGK, from the internal region of the protein sequence according to NP_009292.1; NP_000336.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.
Species Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Specific References
This antibody has been successfully used in the following paper:
Karina J. Vargas, P. L. Colosi, Eric Girardi, Jae-Min Park, Sreeganga S. Chandra
α-Synuclein facilitates clathrin assembly in synaptic vesicle endocytosis
(2021) https://doi.org/10.1101/2020.04.29.069344
PMID: 0
This antibody has been successfully used in Western blot on Human:
Sandro Huenchuguala & Birgitta Sjödin & Bengt Mannervik & Juan Segura-Aguilar
Novel Alpha-Synuclein Oligomers Formed with the Aminochrome-Glutathione Conjugate Are Not Neurotoxic
Neurotox Res. 2019 Feb;35(2):432-440.
PMID: 30343424
Product Reviews
Please login to review this product.- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol