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Code Name Applications Availability Size Tested species Price Grade  
EB07069 Goat Anti-GAPDH (Internal) Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB07069 1
EB07069 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07069 2
EB07069 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07069 3
EB07069 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase, epididymis secretory sperm binding protein Li 162eP, HEL-S-162eP, peptidyl-cysteine S-nitrosylase GAPDH
Official Symbol: GAPDH
Accession Number(s): NP_002037.2; NP_001243728.1
Human GeneID(s): 2597
Non-Human GeneID(s): 14433 (mouse), 24383 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.


Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2; NP_001243728.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 36kDa band observed in Human Liver, Testis and Tonsil lysates (calculated MW of 36.1kDa according to NP_002037.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in Western blot in Human:
Carr AJ, Vugler AA, Yu L, Semo M, Coffey P, Moss SE, Greenwood J.
The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide.
Mol Vis. 2011;17:1701-15.
PMID: 21738400

This antibody has been successfully used in Western blot in Human:
Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ.
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Mol Vis. 2009;15:283-95.
PMID: 19204785

This antibody has been successfully used in the following paper:
Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P.
Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation.
Exp Neurol. 2008 Dec;214(2):347-61.
PMID: 18926821

This antibody has been successfully used in Western blot in Dog:
Chakir K, Daya SK, Aiba T, Tunin RS, Dimaano VL, Abraham TP, Jaques-Robinson KM, Lai EW, Pacak K, Zhu WZ, Xiao RP, Tomaselli GF, Kass DA.
Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.
Circulation. 2009 Mar 10;119(9):1231-40.
PMID: 19237665

This antibody has been successfully used in Western blot in Human:
Schillace RV, Miller CL, Pisenti N, Grotzke JE, Swarbrick GM, Lewinsohn DM, Carr DW.
A-kinase anchoring in dendritic cells is required for antigen presentation.
PLoS One. 2009;4(3):e4807.
PMID: 19277197

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