Search
Type in product code or name:
Goat Anti-GAPDH (Internal) Antibody (EB07069)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07069 | Goat Anti-GAPDH (Internal) Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Dog | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
|
||||||||
Target Protein
Principal Names: glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, OTTHUMP00000174432, OTTHUMP00000174431, MGC88685, GAPD, G3PD, HGNC:4141, glyceraldehyde-3-phosphate dehydrogenase, GAPDHOfficial Symbol: GAPDH
Accession Number(s): NP_002037.2
Human GeneID(s): 2597
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Immunogen
Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:4000.Western blot: Approx 36kDa band observed in Human Liver, Testes and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1kDa according to NP_002037.2). Recommended concentration: 0.1-0.3µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog
Specific References
This antibody has been successfully used in the following papers:
Carr AJ, Vugler AA, Yu L, Semo M, Coffey P, Moss SE, Greenwood J.
The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide.
Mol Vis. 2011;17:1701-15.
PMID: 21738400
Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ.
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Mol Vis. 2009;15:283-95.
PMID: 19204785
Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P.
Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and
retinal transplantation.
Exp Neurol. 2008 Dec;214(2):347-61.
PMID: 18926821
The goat polyclonal antibody used in the following papers was manufactured by us:
Chakir K, Daya SK, Aiba T, Tunin RS, Dimaano VL, Abraham TP, Jaques-Robinson KM, Lai EW, Pacak K, Zhu WZ, Xiao RP, Tomaselli GF, Kass DA.
Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.
Circulation. 2009 Mar 10;119(9):1231-40.
PMID: 19237665
Schillace RV, Miller CL, Pisenti N, Grotzke JE, Swarbrick GM, Lewinsohn DM, Carr DW.
A-kinase anchoring in dendritic cells is required for antigen presentation.
PLoS One. 2009;4(3):e4807.
PMID: 19277197