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Code Name Applications Availability Size Tested species Price Grade  
EB07174 Goat Anti-AIF1 / IBA1 (isoform 3) Antibody Pep-ELISA, WB, IF, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07174 1
EB07174 (0.5µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07174 2
EB07174 (2µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07174 3
EB07174 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07174 4
EB07174 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
Official Symbol: AIF1
Accession Number(s): NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse)
Important Comments: This antibody is expected to recognise isoform 3 (NP_001614.3) only.

Immunogen

Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration: 2-4µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

Specific Reference

This antibody (previous batch) has been successfully used in IHC on Rat:
Joshua P. Thaler, Chun-Xia Yi, Ellen A. Schur, Stephan J. Guyenet, Bang H. Hwang, Marcelo O. Dietrich, Xiaolin Zhao, David A. Sarruf, Vitaly Izgur, Kenneth R. Maravilla, Hong T. Nguyen, Jonathan D. Fischer, Miles E. Matsen, Brent E. Wisse, Gregory J. Morton, Tamas L. Horvath, Denis G. Baskin, Matthias H. Tschöp, and Michael W. Schwartz
Obesity is associated with hypothalamic injury in rodents and humans.
J Clin Invest. 2012 Jan 3; 122(1): 153-162.
PMID: 22201683

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