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Goat Anti-ALOX15 Antibody (EB09049)

Code Name Applications Availability Size Tested species Price Grade  
EB09049 Goat Anti-ALOX15 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $341.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB09049 1
EB09049 (0.2µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09049 2
EB09049 (2µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09049 3
EB09049 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2
Official Symbol: ALOX15
Accession Number(s): NP_001131.3
Human GeneID(s): 246

Immunogen

Peptide with sequence C-HYKTDVAVKDDPE, from the internal region of the protein sequence according to NP_001131.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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