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Goat Anti-APOL4 (C Terminus) Antibody (EB08768)

Related Products: EBP08768 - Immunizing Peptide, EBP09751 - Immunizing Peptide, Goat Anti-APOL4 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08768 Goat Anti-APOL4 (C Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08768 1
EB08768 (0.5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
EB08768 2
EB08768 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: apolipoprotein L4, APOL4, apolipoprotein L, 4, APOL-IV, APOLIV, OTTHUMP00000028937, apolipoprotein L-IV
Official Symbol: APOL4
Accession Number(s): NP_085146.2; NP_663693.1
Human GeneID(s): 80832
Important Comments: This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).

Immunogen

Peptide with sequence C-ENLNELTHIHQSLK, from the C Terminus of the protein sequence according to NP_085146.2; NP_663693.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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