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Goat Anti-Ascl3 (mouse) Antibody (EB10633)

Related Products: EBP10633 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10633 Goat Anti-Ascl3 (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10633 1
EB10633 (0.1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10633 2
EB10633 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: Ascl3, achaete-scute complex homolog 3 (Drosophila), MGC129412, MGC129413, Sgn1, bHLHa42, ASH-3, ICRFP703B1614Q5.4, ICRFP703N2430Q5.4, achaete-scute homolog 3, bHLH transcriptional regulator Sgn-1, mASH-3, mASH3
Official Symbol: Ascl3
Accession Number(s): NP_064435.1
Human GeneID(s): 56676
Non-Human GeneID(s): 56787 (mouse)

Immunogen

Peptide with sequence C-HLPEDYLEKRLSK, from the internal region of the protein sequence according to NP_064435.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

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