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Code Name Applications Availability Size Tested species Price Grade  
EB07671 Goat Anti-ATGL / Desnutrin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07671 1
EB07671 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07671 2
EB07671 (2.5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATGL, DESNUTRIN, PNPLA2, patatin-like phospholipase domain containing 2, 1110001C14Rik, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2, adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase
Official Symbol: PNPLA2
Accession Number(s): NP_065109.1
Human GeneID(s): 57104
Non-Human GeneID(s): 66853 (mouse), 361676 (rat)
Important Comments: Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.

Immunogen

Peptide with sequence C-NIIEVSKEARKR, from the internal region of the protein sequence according to NP_065109.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.
IHC: In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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