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Goat Anti-BDH2 / DHRS6 (aa 60 to 71) Antibody (EB08290)

Related Products: EBP08290 - Immunizing Peptide, EBP08340 - Immunizing Peptide, Goat Anti-BDH2 / DHRS6 (aa 92 to 103) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08290 Goat Anti-BDH2 / DHRS6 (aa 60 to 71) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08290 1
EB08290 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08290 2
HEK293 overexpressing BDH2 (RC210586) and probed with EB08290 (mock transfection in first lane), tested by Origene.
EB08290 3
EB08290 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BDH2, DHRS6, 3-hydroxybutyrate dehydrogenase, type 2, EFA6R, FLJ13261, PRO20933, UCPA-OR, UNQ6308, dehydrogenase/reductase (SDR family) member 6, oxidoreductase UCPA
Official Symbol: BDH2
Accession Number(s): NP_064524.3
Human GeneID(s): 56898

Immunogen

Peptide with sequence C-TKKKQIDQFANE, from the internal region of the protein sequence according to NP_064524.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used in Western Blot on Human:
Buhrke T, Lengler I, Lampen A.
Analysis of proteomic changes induced upon cellular differentiation of the human intestinal cell line Caco-2.
Dev Growth Differ. 2011 Apr;53(3):411-26
PMID: 21492153

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