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Goat Anti-BRM Antibody (EB08634)

Code Name Applications Availability Size Tested species Price Grade  
EB08634 Goat Anti-BRM Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB08634 1
EB08634 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08634 2
EB08634 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing predominantly nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2, BAF190, BRM, FLJ36757, MGC74511, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a, SNF2-alpha, SNF2-like 2, SNF2/SWI2-like protein 2, SWI/SNF-re
Official Symbol: SMARCA2
Accession Number(s): NP_003061.3; NP_620614.2
Human GeneID(s): 6595
Important Comments: This antibody is expected to recognise both reported isoforms (NP_003061.3; NP_620614.2)

Immunogen

Peptide with sequence C-PSNSQLEIEGNSS, from the internal region of the protein sequence according to NP_003061.3; NP_620614.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.

Immunofluorescence: Strong expression of the protein seen in the Nucleus and some staining in the Cytoplasm of HeLa and U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat

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