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Goat Anti-Catalase / CAT Antibody - Trial (EB07282-T)

Related Products: EBP07282 - Immunizing Peptide, Goat Anti-Catalase / CAT Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07282-T Goat Anti-Catalase / CAT Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
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TestShot
EB07282-T 1
EB07282 (0.01µg/ml) staining of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07282-T 2
EB07282 (4µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07282-T 3
EB07282 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CAT, catalase, HGNC:1516, MGC138422, MGC138424
Official Symbol: CAT
Accession Number(s): NP_001743.1
Human GeneID(s): 847
Non-Human GeneID(s): 12359 (mouse)

Immunogen

Peptide with sequence C-DKYNAEKPKNAIHT, from the internal region of the protein sequence according to NP_001743.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in Human and Mouse Kidney and Liver lysates (calculated MW of 59.8kDa according to Human NP_001743.1 and Mouse NP_033934.2 ). Recommended concentration: 0.003-0.01µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

Specific References

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Baret P, Le Sage F, Planesse C, Meilhac O, Devin A, Bourdon E, Rondeau P
Glycated human albumin alters mitochondrial respiration in preadipocyte 3T3-L1 cells.
Biofactors. 2017 Jul 8;43(4):577-592.
PMID: 28543688

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Diotel N, Guerin-Dubourg A, Baret P, Bravo SB, Paradela-Dobarro B, Álvarez E, Essop MF, Meilhac O, Bourdon E, Rondeau P.
Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin.
Free Radic Biol Med. 2017 Jan;102:133-148.
PMID: 27890722

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