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Goat Anti-CBX1 / HP1-Beta Antibody (EB06958)

Related Products: EBP06958 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06958 Goat Anti-CBX1 / HP1-Beta Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06958 1
EB06958 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06958 2
EB06958 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CBX1, HP1-BETA, chromobox homolog 1 (HP1 beta homolog Drosophila ), HGNC:1551, CBX, HP1Hs-beta, M31, MOD1, chromobox homolog 1 (Drosophila HP1 beta), heterochromatin protein p25 beta, HP1Hsbeta, p25beta, heterochromatin protein 1-beta, modifier 1 protein
Official Symbol: CBX1
Accession Number(s): NP_006798.1; NP_001120700.1
Human GeneID(s): 10951
Non-Human GeneID(s): 12412 (mouse), 360609 (rat)
Important Comments: Variants (NP_006798.1; NP_001120700.1) encode the same protein.

Immunogen

Peptide with sequence C-QSQKTAHETDKSE, from the internal region of the protein sequence according to NP_006798.1; NP_001120700.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 25kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25kDa according to NP_006798.1). Recommended concentration: 0.3-1.0 µg/ml.
IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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