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|EB06621||Goat Anti-CD274 / PD-L1 Antibody||Pep-ELISA, WB, IHC, IF, FC||100µg specific antibody in 200µl||Human||$315.00|
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Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
This antibody has been successfully used in Western blot in Human:
Guozhi Xia, Xiaopu Zheng, Xinye Yao, Xiaowei Yao, Zhongwei Liu, Junkui Wang.
Expression of programmed cell death-1 and its ligand B7 homolog 1 in peripheral blood lymphocytes from patients with peripartum cardiomyopathy.
Clin Cardiol. 2016 Dec 27.
This antibody has been successfully used in Western blot and IHC in Human:
Chen J, Li G, Meng H, Fan Y, Song Y, Wang S, Zhu F, Guo C, Zhang L, Shi Y.
Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas.
Cancer Immunol Immunother. 2012 Jan;61(1):101-8.