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Goat Anti-CD274 / PD-L1, Biotinylated Antibody (EB06621-B)

Code Name Applications Availability Size Tested species Price Grade  
EB06621-B Goat Anti-CD274 / PD-L1, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06621-B 1
Biotinylated EB06621 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD274, CD274 molecule, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, CD274 antigen, PDCD1 ligand 1
Official Symbol: CD274
Accession Number(s): NP_054862.1; NP_001254635.1
Human GeneID(s): 29126
Important Comments: This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1) only.

Immunogen

Peptide with sequence CKKQSDTHLEET., from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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