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Goat Anti-CGI58 / ABHD5 Antibody (EB07657)

Related Products: EBP07657 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07657 Goat Anti-CGI58 / ABHD5 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07657 1
EB07657 (0.2µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07657 2
EB07657 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07657 3
EB07657 (3.75µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07657 4
EB07657 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
Official Symbol: ABHD5
Accession Number(s): NP_057090.2
Human GeneID(s): 51099
Non-Human GeneID(s): 67469 (mouse), 316122 (rat)
Important Comments: This antibody is not expected to cross-react with ABHD4.

Immunogen

Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1kDa according to NP_057090.2). Additional faint bands of 26kDa and 18kDa were also observed, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.2-0.6µg/ml Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Cow:
Koltes DA, Spurlock DM.
Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows.
J Dairy Sci. 2011 Apr;94(4):1839-48.
PMID: 21426973

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