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Goat Anti-CHFR Antibody (EB05819)

Related Products: EBP05819 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05819 Goat Anti-CHFR Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05819 1
EB05819 (2.5µg/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196
Official Symbol: CHFR
Accession Number(s): NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1
Human GeneID(s): 55743
Non-Human GeneID(s): 231600 (mouse), 288734 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms.

Immunogen

Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693.
IHC: In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig Cow

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