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Goat Anti-CHRNA4 (aa29-43) Antibody (EB08852)

Code Name Applications Availability Size Tested species Price Grade  
EB08852 Goat Anti-CHRNA4 (aa29-43) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $333.00 Elite

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EB08852 1
EB08852 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4, cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit
Official Symbol: CHRNA4
Accession Number(s): NP_000735.1
Human GeneID(s): 1137
Non-Human GeneID(s): 11438 (mouse), 25590 (rat)
Important Comments: This antibody is expected NOT to cross-react with the similar alpha 2 subunit.


Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ~75kDa confirmed in both human and rat tissues as the glycoprotein by an anonymous customer.

This antibody was shown unfit for immune precipitation.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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