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Goat Anti-Chromogranin A precursor Antibody - Trial (EB07271-T)

Related Products: EBP07271 - Immunizing Peptide, Goat Anti-Chromogranin A precursor Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07271-T Goat Anti-Chromogranin A precursor Antibody - Trial Pep-ELISA, WB, FC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07271-T 1
EB07271 (1µg/ml) staining of Jurkat (A) and LNCaP (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07271-T 2
EB07271 (0.3µg/ml) staining of Human Cerebellum (A) and (1ug/ml) Kidney (B), Pancreas iso 1 © and Pancreas iso 2 (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07271-T 3
EB07271 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: chromogranin A precursor, chromogranin A (parathyroid secretory protein 1), CHGA, CGA, parathyroid secretory protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I, SP1
Official Symbol: CHGA
Accession Number(s): NP_001266.1; NP_001288619.1
Human GeneID(s): 1113

Immunogen

Peptide with sequence C-QGAKERAHQQKKH, from the internal region of the protein sequence according to NP_001266.1; NP_001288619.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 50kDa band observed in lysates of cell lines Jurkat and LNCaP, and in Human Cerebellum, Pancreas and Kidney lysates. A band at 35kDa was also observed in pancreas lysate (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 and 34.3kDa according to NP_001288619.1 isoform 2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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