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Goat Anti-CLPP Antibody (EB05873)

Related Products: EBP05873 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05873 Goat Anti-CLPP Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05873 1
EB05873 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05873 2
EB05873 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CLPP, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent proteolytic subunit E.coli homolog, ATP-dependent protease ClpAP (E. coli), proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli) homolog, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, caseinolytic peptidase, ATP-dependent, proteolytic subunit
Official Symbol: CLPP
Accession Number(s): NP_006003.1
Human GeneID(s): 8192

Immunogen

Peptide with sequence C-EAAPAAEPVPAST, from the C Terminus of the protein sequence according to NP_006003.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 26kDa band observed in Human Heart (calculated MW of 30.2kDa according to NP_006003.1). Recommended for use at 1-3µg/ml.
IHC: In paraffin embedded Human Liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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