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Goat Anti-CMA1 Antibody (EB08876)

Related Products: EBP08876 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08876 Goat Anti-CMA1 Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08876 1
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with EB08876 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB08876(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB08876 2
EB08876 (1.5µg/ml) staining of paraffin embedded Human Testis (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08876 3
EB08876 (1.5µg/ml) staining of paraffin embedded Human Tonsil (Mast cells). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CMA1, chymase 1, mast cell, CYH, MCT1, MGC119890, MGC119891, chymase 1 preproprotein transcript E, chymase 1 preproprotein transcript I, chymase, heart, chymase, mast cell, mast cell protease I
Official Symbol: CMA1
Accession Number(s): NP_001827.1
Human GeneID(s): 1215

Immunogen

Peptide with sequence C-QFRHPKYNTSTLHHD, from the internal region of the protein sequence according to NP_001827.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1µg/ml.
IHC: Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5µg/ml.

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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