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Goat Anti-COX1 / PTGS1 Antibody - Trial (EB05285-T)

Related Products: EBP05285 - Immunizing Peptide, Goat Anti-COX1 / PTGS1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05285-T Goat Anti-COX1 / PTGS1 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05285-T 1
EB05285 (1µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05285-T 2
EB05285 (3.8µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Official Symbol: PTGS1
Accession Number(s): NP_000953.2; NP_542158.1
Human GeneID(s): 5742
Important Comments: This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.

Immunogen

Peptide with sequence C-QDDGPAVERPSTEL, from the C Terminus of the protein sequence according to NP_000953.2; NP_542158.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in lysates of histiocytic lymphoma cell line U937 (calculated MW of 68.7kDa according to NP_000953.2) Recommended for use at 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Heart shows patchy staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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