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Goat Anti-Creatine transporter 1 / SLC6A8 Antibody (EB10642)

Related Products: EBP10642 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10642 Goat Anti-Creatine transporter 1 / SLC6A8 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse, Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10642 1
EB10642 (0.1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10642 2
EB10642 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC6A8, solute carrier family 6 (neurotransmitter transporter, creatine), member 8, CRT, CRTR, CT1, MGC87396, creatine transporter 1, creatine transporter SLC6A8, sodium- and chloride-dependent creatine transporter 1
Official Symbol: SLC6A8
Accession Number(s): NP_005620.1; NP_001136277.1; NP_001136278.1
Human GeneID(s): 6535
Non-Human GeneID(s): 102857 (mouse), 50690 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_005620.1; NP_001136277.1; NP_001136278.1).

Immunogen

Peptide with sequence ASLANLTCDQLADRR, from the internal region of the protein sequence according to NP_005620.1; NP_001136277.1; NP_001136278.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 70kDa band observed in Mouse Heart lysates (calculated MW of 71.0kDa according to Mouse NP_598748.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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