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Code Name Applications Availability Size Tested species Price Grade  
EB12710 Goat Anti-CSNK2B Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12710 1
EB12710 (1µg/ml) staining of NIH3T3 (A), HeLa (B), K562 (C), MCF7 (D) and HepG2 (E) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12710 2
EB12710 (0.3µg/ml) staining of Mouse (A), Rat (B) and Pig (C) Testis lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CSNK2B, casein kinase 2, beta polypeptide, CK2B, CK2N, CSK2B, G5A, CK II beta, Casein kinase II beta subunit, alternative name: G5a, phosvitin, casein kinase II subunit beta, phosvitin, protein G5a
Official Symbol: CSNK2B
Accession Number(s): NP_001311.3; NP_001269314.1
Human GeneID(s): 1460
Non-Human GeneID(s): 13001 (mouse), 81650 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001311.3; NP_001269314.1).

Immunogen

Peptide with sequence C-TGLNEQVPHYRQ, from the internal region of the protein sequence according to NP_001311.3; NP_001269314.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 26kDa band observed in lysates of cell lines NIH3T3, HeLa, K562, MCF7 and HepG2 (calculated MW of 24.9kDa according to NP_001311.3). Approx 25kDa band observed in Mouse Testis, 26kDa in Rat Testis and 28kDa in Pig Testis lysates (calculated MW of 24.9kDa according to Mouse NP_001290374.1, Rat NP_001030315.1 and pig NP_001138849.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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