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Goat Anti-Cyld (mouse) Antibody (EB07719)

Related Products: EBP07719 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07719 Goat Anti-Cyld (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Rat, Mouse $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07719 1
EB07719 (0.5µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07719 2
EB07719 (5µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CYLD, cylindromatosis (turban tumor syndrome), 2010013M14Rik, 2900009M21Rik, C130039D01Rik, CDMT, CYLD1, EAC, mKIAA0849, ubiquitin carboxyl-terminal hydrolase CYLD
Official Symbol: Cyld
Accession Number(s): NP_775545.1
Non-Human GeneID(s): 74256 (mouse), 312937 (rat)

Immunogen

Peptide with sequence C-DSQQSKSKNPWYIDE, from the internal region of the protein sequence according to NP_775545.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 125kDa band observed in Rat Brain lysates (calculated MW of 107kDa according to mouse NP_775545.1). Recommended concentration: 0.5-1.5µg/ml.
IHC: Paraffin embedded Mouse Brain. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Rat, Mouse
Expected from sequence similarity: Mouse, Rat

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