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Goat Anti-CYP26B1 Antibody (EB07481)

Related Products: EBP07481 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07481 Goat Anti-CYP26B1 Antibody Pep-ELISA, FC, IHC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB07481 1
EB07481 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07481 2
EB07481 (4µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
EB07481 3
EB07481 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07481 4
EB07481 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07481 5
EB07481 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: CYP26B1, cytochrome P450, family 26, subfamily B, polypeptide 1, CYP26A2, DKFZp686G0638, MGC129613, P450RAI-2, cytochrome P450 retinoid metabolizing protein, cytochrome P450, family 26, subfamily b, polypeptide 1, retinoic acid-metabolizing cytochrome
Official Symbol: CYP26B1
Accession Number(s): NP_063938.1; NP_001264671.1
Human GeneID(s): 56603
Non-Human GeneID(s): 232174 (mouse), 312495 (rat)

Immunogen

Peptide with sequence C-SQARSEDKDGRFH, from the internal region of the protein sequence according to NP_063938.1; NP_001264671.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration: 4-6µg/ml. Paraffin embedded Human Small Intestine, Spleen and Testis. Recommended concentration: 3.75µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog