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Goat Anti-D-amino-acid oxidase Antibody (EB08319)

Related Products: EBP08319 - Immunizing Peptide, EBP11100 - Immunizing Peptide, Goat Anti-D-amino-acid oxidase (aa286-298) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08319 Goat Anti-D-amino-acid oxidase Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08319 1
EB08319 (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB08319 2
EB08319 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DAO, D-amino-acid oxidase, DAAO, DAMOX, MGC35381, OXDA
Official Symbol: DAO
Accession Number(s): NP_001908.3
Human GeneID(s): 1610

Immunogen

Peptide with sequence C-EEKKLSRMPPSH, from the C Terminus of the protein sequence according to NP_001908.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments in human brain (cerebellum, hippocampus, substantia nigra) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Cerebellum shows pixulated cytoplasm staining in neurons directly near the granular layer. Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Mouse

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