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Goat Anti-DNMT1 Antibody - Trial (EB08317-T)

Related Products: EBP08317 - Immunizing Peptide, Goat Anti-DNMT1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08317-T Goat Anti-DNMT1 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
TestShot
EB08317-T 1
EB08317 (0.5µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08317-T 2
EB08317 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1
Official Symbol: DNMT1
Accession Number(s): NP_001370.1
Human GeneID(s): 1786
Non-Human GeneID(s): 13433 (mouse), 84350 (rat)

Immunogen

Peptide with sequence C-RFESPPKTQPTEDN, from the internal egion of the protein sequence according to NP_001370.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 170kDa band observed in lysates of cell line Jurkat (calculated MW of 183kDa according to NP_001370.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.
IHC: In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration: 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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