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Goat Anti-DUSP8 / HVH5 Antibody (EB05318)

Code Name Applications Availability Size Tested species Price Grade  
EB05318 Goat Anti-DUSP8 / HVH5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05318 1
EB05318 (2µg/ml) staining of Human Cerebellum (A), Mouse (B), and Rat (C) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DUSP8, dual specificity phosphatase 8, HB5, HVH8, HVH-5, H1 phosphatase, vaccinia virus homolog, serine/threonine specific protein phosphatase, C11orf81, FLJ42476, FLJ42958, OTTHUMP00000164487
Official Symbol: DUSP8
Accession Number(s): NP_004411.2
Human GeneID(s): 1850
Non-Human GeneID(s): 18218 (mouse)
Important Comments: This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.

Immunogen

Peptide with sequence AGDRLPRKVMDAK-C, from the N Terminus of the protein sequence according to NP_004411.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 55-58kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 65.8kDa according to Human NP_004411.2, 68.8kDa according to Mouse NP_032774.1 and 66.8kDa according to Rat NP_001101980.1 ). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Cow

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