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Goat Anti-dUTPase / DUT Antibody (EB12748)

Related Products: EBP12748 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12748 Goat Anti-dUTPase / DUT Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12748 1
EB12748 (0.1µg/ml) staining of HEK293 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12748 2
EB12748 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB12748 3
EB12748 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DUT, deoxyuridine triphosphatase, dUTPase, dUTP nucleotidohydrolase, dUTP pyrophosphatase, deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Official Symbol: DUT
Accession Number(s): NP_001020419.1; NP_001939.1; NP_001020420.1
Human GeneID(s): 1854
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001020419.1; NP_001939.1; NP_001020420.1).

Immunogen

Peptide with sequence C-QLRFARLSEHATAPT, from the internal region of the protein sequence according to NP_001020419.1; NP_001939.1; NP_001020420.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Cow

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