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Goat Anti-DYX1C1 / EKN1 Antibody (EB07254)

Code Name Applications Availability Size Tested species Price Grade  
EB07254 Goat Anti-DYX1C1 / EKN1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB07254 1
EB07254 (0.5µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DYX1C1, EKN1, dyslexia susceptibility 1 candidate 1, DYX1, DYXC1, FLJ37882, MGC70618
Official Symbol: DNAAF4
Accession Number(s): NP_570722.2; NP_001028731.1; NP_001028732.1
Human GeneID(s): 161582
Important Comments: This antibody is expected to recognise three reported isoforms (NP_570722.2, NP_001028731.1 and NP_001028732.1).

Immunogen

Peptide with sequence PLQVSDYSWQQTKT-C, from the N Terminus of the protein sequence according to NP_570722.2; NP_001028731.1; NP_001028732.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48+40kDa bands observed in Human Brain (Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 48.5kDa according to NP_570722.2 and of 44.0kDa according to NP_001028731.1). Recommended concentration: 0.5-1.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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