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Goat Anti-ENC1 Antibody (EB11784)

Related Products: EBP11784 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11784 Goat Anti-ENC1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Rat, Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11784 1
EB11784 (1µg/ml) staining of Rat Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11784 2
EB11784 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CCL28, ectodermal-neural cortex (with BTB-like domain), ectodermal-neural cortex 1 (with BTB-like domain), ectoderm-neural cortex protein 1, ENC1, ENC-1, FLJ39259, kelch-like 35, kelch-like 37, kelch-like protein 37, KLHL35, KLHL37, NRPB, nuclear matrix protein NRP/B, nuclear restricted protein, BTB domain-like (brain), OTTHUMP00000128235, OTTHUMP00000222054, p53-induced gene 10 protein, PIG10, TP53I10, tumor protein p53 inducible protein 10
Official Symbol: ENC1
Accession Number(s): NP_003624.1; NP_001243505.1
Human GeneID(s): 8507
Non-Human GeneID(s): 13803 (mouse), 294674 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003624.1; NP_001243505.1). Reported variants represent identical protein: NP_001243503.1, NP_001243504.1, NP_003624.1.

Immunogen

Peptide with sequence TSVSHDKLPKVQC, from the internal region of the protein sequence according to NP_003624.1; NP_001243505.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Rat, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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