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Goat Anti-EPB41L2 / 4.1G (aa 347 to 357) Antibody (EB08171)

Code Name Applications Availability Size Tested species Price Grade  
EB08171 Goat Anti-EPB41L2 / 4.1G (aa 347 to 357) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB08171 1
EB08171 (2.5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08171 2
EB08171 (2.5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: EPB41L2, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, 4.1-G, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2
Official Symbol: EPB41L2
Accession Number(s): NP_001422.1
Human GeneID(s): 2037

Immunogen

Peptide with sequence C-DYDPEEHGSID, from the internal region of the protein sequence according to NP_001422.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Uterus and Breast. Recommended concentration: 2.5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371