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Code Name Applications Availability Size Tested species Price Grade  
EB10171 Goat Anti-ETFB / FP585 (aa 152-165) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $423.00 Elite

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EB10171 1
EB10171 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10171 2
EB10171 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: electron transfer flavoprotein beta subunit, electron transfer flavoprotein beta-subunit, electron transfer flavoprotein, beta polypeptide, electron-transfer-flavoprotein, beta polypeptide, electron-transferring-flavoprotein, beta polyp, FP585, MADD, ETFB
Official Symbol: ETFB
Accession Number(s): NP_001976.1; NP_001014763.1
Human GeneID(s): 2109
Non-Human GeneID(s): 110826 (mouse), 292845 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).

Immunogen

Peptide with sequence C-QVTLEGDKLKVERE, from the internal region of the protein sequence according to NP_001976.1; NP_001014763.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 27kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kDa according to NP_001976.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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