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Goat Anti-ETFB / FP585 (aa 152-165) Antibody (EB10171)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB10171 | Goat Anti-ETFB / FP585 (aa 152-165) Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest |
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EB10171 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10171 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: electron transfer flavoprotein beta subunit, electron transfer flavoprotein beta-subunit, electron transfer flavoprotein, beta polypeptide, electron-transfer-flavoprotein, beta polypeptide, electron-transferring-flavoprotein, beta polyp, FP585, MADD, ETFBOfficial Symbol: ETFB
Accession Number(s): NP_001976.1; NP_001014763.1
Human GeneID(s): 2109
Non-Human GeneID(s): 110826 (mouse), 292845 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).
Immunogen
Peptide with sequence C-QVTLEGDKLKVERE, from the internal region of the protein sequence according to NP_001976.1; NP_001014763.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:8000.Western blot: Approx 27kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kDa according to NP_001976.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow