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Goat Anti-FCER1A (aa177-189) Antibody (EB12554)

Related Products: EBP12554 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12554 Goat Anti-FCER1A (aa177-189) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12554 1
EB12554 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB12554 2
EB12554 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FCER1A, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, FCE1A, FcERI, Fc IgE receptor, alpha polypeptide, Fc epsilon RI alpha-chain, Fc-epsilon RI-alpha, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, igE Fc receptor subunit alpha, immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
Official Symbol: FCER1A
Accession Number(s): NP_001992.1
Human GeneID(s): 2205

Immunogen

Peptide with sequence C-TGKVWQLDYESEP, from the internal region of the protein sequence according to NP_001992.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary testing showed a band at approx. 55kDa in Human Lung lysate, and approx. 50kDa in Human Heart lysate after 3µg/ml antibody staining (calculated MW of 29.6kDa according to NP_001992.1). These molecular weights are observed by other commercial sources, and were blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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