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Goat Anti-GAD1 (isoform GAD67) Antibody (EB09109)

Code Name Applications Availability Size Tested species Price Grade  
EB09109 Goat Anti-GAD1 (isoform GAD67) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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EB09109 1
EB09109 (0.5µg/ml) staining of Human Brain (Cerebral Cortex) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09109 2
EB09109 (4µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: GAD1, glutamate decarboxylase 1 (brain, 67kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25
Official Symbol: GAD1
Accession Number(s): NP_000808.2
Human GeneID(s): 2571
Non-Human GeneID(s): 14415 (mouse), 24379 (rat)
Important Comments: This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.


Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9kDa according to NP_000808.2). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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