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Goat Anti-galectin-3 (mouse) Antibody (EB10775)

Code Name Applications Availability Size Tested species Price Grade  
EB10775 Goat Anti-galectin-3 (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]

EB10775 1
EB10775 (0.5µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10775 2
EB10775 (10µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: Lgals3, lectin, galactose binding, soluble 3, GBP, L-34, Mac-2, gal3, 35 kDa lectin, CBP 35, L-34 galactoside-binding lectin, OTTMUSP00000043689, OTTMUSP00000043690, carbohydrate-binding protein 35, gal-3, galactose-specific lectin 3
Official Symbol: Lgals3
Accession Number(s): NP_034835.1
Non-Human GeneID(s): 16854 (mouse)
Important Comments: Reported variants represent identical protein: NP_001139425.1, NP_034835.1


Peptide with sequence C-NANRIVLDFRRGND, from the internal region of the protein sequence according to NP_034835.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 26kDa band observed in Mouse Heart lysates (calculated MW of 27.4kDa according to NP_034835.1). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Colon. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Mouse

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