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Goat Anti-GCDFP15 / PIP Antibody - Trial (EB07224-T)

Related Products: EBP07224 - Immunizing Peptide, Goat Anti-GCDFP15 / PIP Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07224-T Goat Anti-GCDFP15 / PIP Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07224-T 1
EB07224 (0.3µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07224-T 2
EB07224 (2.5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PIP, GCDFP15, prolactin-induced protein, HGNC:8993, GCDFP-15, GP17, GPIP4, prolactin-inducible protein, gross cystic disease fluid protein 15
Official Symbol: PIP
Accession Number(s): NP_002643.1
Human GeneID(s): 5304

Immunogen

Peptide with sequence C-GANKAQDNTRK, from the internal region of the protein sequence according to NP_002643.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Breast shows staining of luminal epithelial cells of the lactiferous duct. Recommended concentration: 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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