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Goat Anti-GIPC1 / NIP Antibody (EB06196)

Related Products: EBP06196 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06196 Goat Anti-GIPC1 / NIP Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB06196 1
EB06196 staining (0.03µg/ml) of Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB06196 2
HEK293 overexpressing GIPC1 isoform 1 (RC216466) and probed with EB06196 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: GIPC1, NIP, GIPC PDZ domain containing family, member 1, C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, MGC15889, MGC3774, RGS19IP1, SEMCAP, SYNECTIIN, TIP-2, GLUT1 C-terminal binding protein, IGF-1 receptor interacting protein 1, regulator of G-protein signalling 19 interacting protein 1, synectin, SYNECTIN
Official Symbol: GIPC1
Accession Number(s): NP_005707.1; NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1
Human GeneID(s): 10755
Non-Human GeneID(s): 67903 (mouse), 83823 (rat)
Important Comments: This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).

Immunogen

Peptide with sequence C-GAIGDAKVGRY, from the C Terminus of the protein sequence according to NP_005707.1; NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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