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Goat Anti-Glutathione Peroxidase 1 (isoform1) Antibody - Trial (EB07283-T)

Related Products: EBP07283 - Immunizing Peptide, Goat Anti-Glutathione Peroxidase 1 (isoform1) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07283-T Goat Anti-Glutathione Peroxidase 1 (isoform1) Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Pig $105.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07283-T 1
EB07283 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07283-T 2
EB07283 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07283-T 3
EB07283 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07283-T 4
EB07283 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia
Click here to download PDF datasheet.

Target Protein

Principal Names: glutathione peroxidase 1, HGNC:4553, GPX1, GSHPX1, MGC14399, MGC88245
Official Symbol: GPX1
Accession Number(s): NP_000572.2
Human GeneID(s): 2876
Important Comments: This antibody is expected to recognise isoform 1 ( NP_000572.2) only.

Immunogen

Peptide with sequence C-REALPAPSDDATA, from the internal region of the protein sequence according to NP_000572.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 22kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5µg/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75µg/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration: 20µg/ml.

Species Reactivity

Tested: Human, Pig
Expected from sequence similarity: Human

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