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Goat Anti-HIF1alpha / MOP1 Antibody (EB10789)

Code Name Applications Availability Size Tested species Price Grade  
EB10789 Goat Anti-HIF1alpha / MOP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB10789 1
EB10789 (0.3µg/ml) staining of Human Kidney (A), Lung (B) and Gastrointestinal cancer (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
EB10789 2
EB10789 (0.5µg/ml) staining of Mouse Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: HIF1A, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, ARNT interacting protein, HIF-1-alpha, OTTHUMP00000179063, PAS domain-containing
Official Symbol: HIF1A
Accession Number(s): NP_001521.1; NP_851397.1
Human GeneID(s): 3091
Non-Human GeneID(s): 15251 (mouse), 29560 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001521.1; NP_851397.1). This antibody is NOT expected to cross-react with EPAS1 (GeneID 2034).

Immunogen

Peptide with sequence C-KMTQLFTKVESED, from the internal region of the protein sequence according to NP_001521.1; NP_851397.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 90kDa band observed in Human and Mouse Lung and in Human Kidney lysates and approx 85-90kDa in Human Gastrointestinal cancer lysates (calculated MW of 92.7kDa according to Human NP_001521.1, 82.7kDa according to Human NP_851397.1 and 93.7kDa according to Mouse NP_001300848.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human. Mouse, Rat, Dog, Pig, Cow

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