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Goat Anti-Histamine Receptor H2 (aa309-323) (mouse) Antibody (EB11020)

Code Name Applications Availability Size Tested species Price Grade  
EB11020 Goat Anti-Histamine Receptor H2 (aa309-323) (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $423.00 Elite

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EB11020 1
EB11020 (0.5µg/ml) staining of Mouse Stomach lysate (A) and (2µg/ml) Rat Small Intestine lysate (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Target Protein

Principal Names: HRH2, histamine receptor H2, H2R, HH2R, gastric receptor 1, gastric receptor I, histamine H2 receptor, gastric receptor 1, gastric receptor I, H2R, HH2R, histamine H2 receptor, histamine receptor H2, HRH2
Official Symbol: Hrh2
Accession Number(s): NP_001399939.1; NP_001010973.1
Non-Human GeneID(s): 15466 (mouse), 25461 (rat)
Important Comments: This antibody is expected to recognize Mouse NP_001399939.1 iso 3, NP_001010973.1 iso 1 and Rat NP_001010973.1.

Immunogen

Peptide with sequence C-SHNSHKTSLRLNNS, from the internal region of the protein sequence according to NP_001399939.1; NP_001010973.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 50kDa band observed in Mouse Stomach and Rat Small Intestine lysates (calculated MW of 47.3kDa according to Mouse NP_001399939.1 and 45.2kDa according to Rat NP_001399940.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat